In the present study, we have included DR4:A>C (rs20576), G>A (rs6557634); FAS-1377G>A (rs2234767); FASL-844T>C (rs763110); DCC:C>G (rs2229080), A>G (rs4078288), C>T (rs7504990), A>G (rs714); PSCA:C>T (rs2294008), G>A (rs2978974); ADRA2A-1291C>G (rs1801253); ADRB1 1165C>G (rs1800544); ADRB3 190T>C (rs4994); and CYP17 T>C (rs2486758) SNPs.
Salting out method was used to isolate genomic DNA from 5 mL peripheral blood leukocytes [60 (link)]. The genotyping was performed by the PCR restriction fragment length polymorphism and TaqMan® allelic discrimination assays (Applied Biosystems 7500 Fast Real-Time PCR (Thermo Fisher Scientific, Walthan, MA, USA)) method, as described previously [8 (link),9 (link),10 (link),11 (link),12 (link)]. PCR mix without DNA sample was taken as negative control and the 10% of random samples were sequenced to confirm the results consistency.
Salting out method was used to isolate genomic DNA from 5 mL peripheral blood leukocytes [60 (link)]. The genotyping was performed by the PCR restriction fragment length polymorphism and TaqMan® allelic discrimination assays (Applied Biosystems 7500 Fast Real-Time PCR (Thermo Fisher Scientific, Walthan, MA, USA)) method, as described previously [8 (link),9 (link),10 (link),11 (link),12 (link)]. PCR mix without DNA sample was taken as negative control and the 10% of random samples were sequenced to confirm the results consistency.
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