Extraction and Quantification of Bioactive Compounds

Methanol (HPLC grade) was obtained from Merck (Darmstadt, Germany). Formic acid, ethanol, n-butanol, and Tween®-80 were obtained from Synth (Diadema, Brazil). Vitexin and isoVitexin standards (99.99%) were purchased from Sigma-Aldrich (São Paulo, Brazil). The 6-C-glycoside-diosmetin and vicenin-2 standards were generated in our laboratory according to the methods described by de Oliveira et al. (2014 (link)). Valium® (diazepam) was purchased from Roche (São Paulo, Brazil). Sintocalmy® (standardized extract of Passiflora incarnate L.—extract ACH 06) was obtained from Aché (Guarulhos, Brazil). Ro25-6981, picrotoxin and (S)-WAY100135 were purchased from Tocris Biosciences (Ellisville, MO, USA). NMDA was obtained from Sigma-Aldrich (São Paulo, Brazil). Buspirone hydrochloride was obtained from LIBBS Pharmaceutical Ltd (São Paulo, Brazil).