DRG Cl- Measurement via MQAE

L_4–6 DRG specimens were collected from different groups. In accordance with the experimental procedure of Batti et al. (2013) (link) and the improvement methods from the authors’ laboratory (Tan et al., 2020 (link)), the animals were sacrificed using 4% sevoflurane, with immediate stripping of L_4–6 DRG. Then, samples were cut with eye scissors, subjected to enzyme digestion, and centrifuged. After the clear liquid was removed, the specimens were placed on a 12-well plate that had been precoated with poly-D-lysine (Beyotime) for fixing cells. Then, N-ethoxycarbonylmethyl-6-methoxyquinolinium bromide (MQAE) buffer was added and incubated at 37°C for 30 min, and the specimens were cleaned with a buffer. A fluorescence microscope was used to record the fluorescence intensity. When the concentration of Cl^– in the cell increased, the fluorescence intensity of MQAE was proportionally reduced.