Gas Chromatography-Mass Spectrometry Analysis

Gas chromatography with Agilent 7890A/5975C mass spectrometric detection was used to conduct the extract composition analysis. Chromatography conditions were as follows: sample 1.0 μl, sample input temperature 260°C, and no flow splitting. Separation was performed using a DB-WAXetr chromatographic capillary column with a 30 m length, an inner diameter of 0.25 mm, and a film thickness of 0.25 μm at a constant carrier gas rate (helium) of 1 ml/min. [13 ]. The chromatography temperature is programmed from 40°C (10 min time of exposure) at a heating rate of 5°C/min to 270°C (10 min exposure). Detection is performed in scan mode m/z 34–750. Agilent MSD ChemStation software (version 1701EA) was used to control the gas chromatography system, record, and process the results and data [14 (link)]. The chromatogram obtained was analysed, and each peak was checked by determining the percent area on the chromatogram, the retention time, the spectrum, and the base peak and then referring to the characteristic mass spectra of compounds listed.