Annexin V Apoptosis Detection Assay

The Annexin V In Situ Cell Apoptosis Detection Kit (Roche, Indianapolis, IN) [61 , 63 (link)] was employed to test cell apoptosis. In brief, following the applied LB-100 treatment, cells were washed, fixed and stained with Annexin V (2.5 μg/mL) and propidium iodide (PI) (2.5 μg/mL). The cell apoptosis ratio was reflected by Annexin V^+/+/PI^-/- plus Annexin V^+/+/PI^+/+ percentage detected by fluorescence-activated cell sorting (FACS) counter (Beckman Coulter, Shanghai, China).

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Dai C., Zhang X., Xie D., Tang P., Li C., Zuo Y., Jiang B, & Xue C. (2017). Targeting PP2A activates AMPK signaling to inhibit colorectal cancer cells. Oncotarget, 8(56), 95810-95823.

Publication 2017

Annexin V In Situ Cell Apoptosis Detection Kit

Annexin Annexin v Apoptosis Cell Lb 100 Propidium iodide

Corresponding Organization :

Other organizations : Jiangsu Food and Pharmaceutical Science College, Second Affiliated Hospital of Nanjing Medical University, Nantong University, Huaian First People’s Hospital

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Variable analysis

independent variables

LB-100 treatment

dependent variables

Cell apoptosis ratio reflected by Annexin V+/+/PI-/- plus Annexin V+/+/PI+/+ percentage detected by fluorescence-activated cell sorting (FACS) counter

control variables

Cell washing
Cell fixation
Staining with Annexin V (2.5 μg/mL) and propidium iodide (PI) (2.5 μg/mL)

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