Characterization of Silica-Coated Magnetic Nanoparticles

MNPs@SiO₂(RITC) particles contain a cobalt ferrite core (CoFe₂O₃) sheathed by a silica shell that is chemically bonded to rhodamine isothiocyanate dye (RITC)49 (link). Silica NPs are identical to MNPs@SiO₂(RITC) except that they lack a cobalt ferrite core and show similar tendencies with respect to their biological effects12 (link)50 (link). MNPs@SiO₂(RITC) and silica NPs are 50 nm in diameter, and the size distribution and zeta-potential of both NPs have been previously reported49 (link)50 (link). A previous study indicated that approximately 10⁵ particles of MNPs@SiO₂(RITC) per cell were taken up in MCF-7 breast cancer cells as determined by inductively coupled plasma atomic emission spectrometry (ICP-AES)49 (link). The dosage used in this study was determined by treating HEK293 cells with MNPs@SiO₂(RITC) at concentrations ranging from 0.01 to 2.0 μg/μl for 12 h and calculating their uptake efficiencies using a fluorescent assessment method12 (link). The optimal concentration of MNPs@SiO₂(RITC) was 0.1 μg/μl for in vitro use and as MRI contrast without toxicological effects in human cord blood-derived mesenchymal stem cells3 (link). Disturbances of gene expression and metabolic profiles at this concentration were similar to those in control HEK293 cells12 (link). The uptake efficiency of MNPs@SiO₂(RITC) plateaued at 1.0 μg/μl. Therefore, a low dose of 0.1 μg/μl and high dose of 1.0 μg/μl were used in the present study.

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Phukan G., Shin T.H., Shim J.S., Paik M.J., Lee J.K., Choi S., Kim Y.M., Kang S.H., Kim H.S., Kang Y., Lee S.H., Mouradian M.M, & Lee G. (2016). Silica-coated magnetic nanoparticles impair proteasome activity and increase the formation of cytoplasmic inclusion bodies in vitro. Scientific Reports, 6, 29095.

Publication 2016

Biological Breast cancer Cell Cobalt ferrite Cord blood Gene expression Hek293 cells Human Mcf 7 cells Mesenchymal Metabolic profiles Plasma Rhodamine isothiocyanate Silica Spectrometry Stem

Corresponding Organization :

Other organizations : Ajou University, Sunchon National University, Seoul National University, Pharmicell (South Korea), Kyung Hee University, Sungkyunkwan University, Rutgers, The State University of New Jersey

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Variable analysis

independent variables

Concentration of MNPs@SiO2(RITC) particles (0.01 to 2.0 μg/μl)
Presence/absence of cobalt ferrite core in the particles (MNPs@SiO2(RITC) vs. silica NPs)

dependent variables

Particle uptake efficiency in HEK293 cells
Disturbances of gene expression and metabolic profiles in HEK293 cells

control variables

Particle size (50 nm diameter)
Particle size distribution
Particle zeta-potential
Incubation time (12 hours)

controls

Positive control: MCF-7 breast cancer cells treated with MNPs@SiO2(RITC) particles
Negative control: HEK293 cells treated with no particles

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