Bone marrow-derived macrophage (BMDM) cells from mice were stimulated with recombinant mouse M-CSF (R&D) as reported previously21 (link). Briefly, the bone marrow cells were stimulated with 20 ng/ml M-CSF. After 7 days culture, more than 90% pure BMDM were obtained. For the following tests, BMDM cells were harvested using a cell scraper (Costar) and cultured at 5 × 105 cells/well in a fresh 24-well plates overnight.
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