Protein Expression Quantification by Flow Cytometry

Protein expression of ALP, OPN, OC, and COL1a1 was evaluated by flow cytometry analysis, following a previous protocol [35 (link)]. Samples were processed using a FACS Canto II flow cytometer (BD Biosciences, San Jose, CA, USA), and data were analyzed using FACSDiva v6.1.3, IDEAS software (BD Biosciences, San Jose, CA, USA), and FlowJo v8.3.3 software (Tree Star Inc., Ashland, OR, USA). Data are indicated as a mean fluorescence intensity (MFI) ratio calculated by dividing the MFI of positive events by the MFI of negative events (MFI of secondary antibody).

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Pelusi L., Mandatori D., Di Pietrantonio N., Del Pizzo F., Di Tomo P., Di Pietro N., Buda R., Genovese S., Epifano F., Pandolfi A., Fiorito S, & Pipino C. (2022). Estrogen Receptor 1 (ESR1) and the Wnt/β-Catenin Pathway Mediate the Effect of the Coumarin Derivative Umbelliferon on Bone Mineralization. Nutrients, 14(15), 3209.

Publication 2022

FACS Canto II FACSDiva v6.1.3 IDEAS software

Flow cytometry Fluorescence Fluorescence antibody Protein Tree

Corresponding Organization : University of Chieti-Pescara

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Variable analysis

independent variables

Protein expression of ALP, OPN, OC, and COL1a1

dependent variables

Mean fluorescence intensity (MFI) ratio calculated by dividing the MFI of positive events by the MFI of negative events (MFI of secondary antibody)

control variables

Samples were processed using a FACS Canto II flow cytometer (BD Biosciences, San Jose, CA, USA)
Data were analyzed using FACSDiva v6.1.3, IDEAS software (BD Biosciences, San Jose, CA, USA), and FlowJo v8.3.3 software (Tree Star Inc., Ashland, OR, USA)

controls

Positive control: Not specified
Negative control: MFI of secondary antibody

Annotations

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