Optokinetic Response in Zebrafish

Adult zebrafish were lightly anaesthetized in 0.2 mg/ml tricaine and placed in a custom-made foam holder supported by dissection pins in a 55 mm petri dish. The dish was filled with tank water and the fish were allowed to regain consciousness, then placed into a custom-made optokinetic device consisting of a 12 cm acrylic drum, rpm adjustable rotating motor with laser tachometer and stereo microscope (Zeiss Stemi-2000C) c-mounted with a digital SLR camera (Nikon D5100). Each zebrafish was assessed with varying grating lengths at a consistent rpm speed (12 rpm) until the stripes could no longer be tracked by the zebrafish, as previously described (52 (link)).