Collagen-Induced Arthritis Model in Mice

Female mice (WT and S100a8^-/- DBA/1) aged 6–8 weeks were immunized subcutaneously at the base of the tail with chicken collagen type II (100 μg/mouse, Chondrex Inc.) emulsified in complete Freund’s adjuvant then 26 days later received an intra-peritoneal injection of LPS (25 μg/mouse). Disease activity was monitored every other day on a scale of 0 to 4 per paw for a maximum score of 16 per mouse as described previously [13 (link)]. For antibody treatment, mice received rabbit polyclonal anti-S100A8 or control IgG three times per week (10 mg/kg of body weight, intra-peritoneal) starting on the day of LPS injection.