Resazurin-Based Viability Assay

A 6× solution of resazurin sodium salt (Sigma–Aldrich, R7017) resuspended in Dulbecco’s phosphate-buffered saline was added to a final concentration of 25 μg/ml in fresh DMEM and then added to multiwell plates. After a 2 h incubation at 37°C, fluorescent signal (560 nm excitation/590 nm emission) was measured using a BioTek Synergy HT plate reader. Values for wells with no cells (i.e. media only) were subtracted for each well prior to further data processing.

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Schillo J.L., Feddersen C.R., Peplinski R.M., Powell L.S., Varzavand A., Stipp C.S., Riordan J.D, & Dupuy A.J. (2024). Single-cell genomics analysis reveals complex genetic interactions in an in vivo model of acquired BRAF inhibitor resistance. NAR Cancer, 6(1), zcad061.

Publication 2024

resazurin sodium salt Synergy HT

Corresponding Organization :

Other organizations : University of Iowa

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Variable analysis

independent variables

Concentration of resazurin sodium salt solution

dependent variables

Fluorescent signal (560 nm excitation/590 nm emission)

control variables

Cell culture medium (DMEM)
Incubation time (2 h)
Incubation temperature (37°C)
Plate reader (BioTek Synergy HT)

controls

Positive control: Not explicitly mentioned.
Negative control: Wells with no cells (i.e., media only)

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