PDX tumor samples were processed using Tissue-Tek optimum cutting temperature (OCT) compound (Sakura Finetek) and frozen in liquid nitrogen prior to being cut into slides by the University of Colorado Cancer Center (UCCC) Pathology Core Facility. Slides were fixed and stained for SA-β-gal activity at pH of 6.0 using the Senescence β-gal Staining Kit (Cell Signaling Technology), as described previously (14 (link)). Hematoxylin and eosin (H&E) staining was performed on matched slides by the UCCC Pathology Core Facility and reviewed by a pathologist, confirming the presence of tumor cells. Representative images were acquired using a Zeiss microscope at 40 × magnification.
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Senescence Assay for PDX Tumors
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Corresponding Organization : University of Colorado Cancer Center
Other organizations : University of California, Irvine, University of Colorado Boulder, University of Alabama at Birmingham, Moffitt Cancer Center, The University of Texas at Austin, Colorado State University
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- Processing of PDX tumor samples using Tissue-Tek optimum cutting temperature (OCT) compound and freezing in liquid nitrogen
- Senescence-associated beta-galactosidase (SA-β-gal) activity at pH 6.0
- Presence of tumor cells confirmed by hematoxylin and eosin (H&E) staining
- Slides were fixed and stained for SA-β-gal activity using the Senescence β-gal Staining Kit (Cell Signaling Technology)
- H&E staining was performed on matched slides by the UCCC Pathology Core Facility and reviewed by a pathologist
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