Quantifying Ethylene Production in Mangoes

The production of ethylene was determined following the procedures described by Chen et al. with minor modifications (Chen et al., 2022 (link)). Three mango fruit were weighed and sealed in a 12 l airtight jar for 2 h at 25°C. Three 1 ml gas samples were collected from each vessel by syringe and injected into a gas chromatograph (Agilent 5,181–1,267, Palo Alto, CA, United States) equipped with a flame ionization detector (Thermo Fisher Scientific, TRACE 1300, United States). The peaks were compared to the standard gas sample of ethylene. Ethylene production was calculated and expressed as μL g⁻¹ h⁻¹.