Methylation Analysis of SLIT2 Promoter

The isolation and modification of genomic DNA was performed using Puregene Blood Core Kit B and EpiTect Bisulfite Kit (QIAGEN, Duesseldorf, Germany) as described previously [15 (link), 16 (link)]. Real-time quantitative methylation-specific PCR (RT-qMSP) was first used to evaluate SLIT2 promoter methylation with AceQ qPCR SYBR Green Master Mix (Vazyme Biotech Co., Piscataway, NJ). The primers for SLIT2 promoter methylation detection were as reported [12 (link)]. Relative SLIT2 promoter methylation was counted using the 2− ^∆∆CT formula as referred to ALU methylation.

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Wu D.L., Wang Y., Zhang T.J., Chu M.Q., Xu Z.J., Yuan Q., Ma J.C., Lin J., Qian J, & Zhou J.D. (2022). SLIT2 promoter hypermethylation predicts disease progression in chronic myeloid leukemia. European Journal of Medical Research, 27, 259.

Publication 2022

Puregene Blood Core Kit B EpiTect Bisulfite Kit AceQ qPCR SYBR Green Master Mix

Bisulfite Blood Genomic Isolation Methylation Primers Real time pcr Sybr green

Corresponding Organization :

Other organizations : Jiangsu University, Affiliated Hospital of Jiangsu University, Shanghai Clinical Research Center

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Variable analysis

independent variables

Genomic DNA isolation and modification using Puregene Blood Core Kit B and EpiTect Bisulfite Kit

dependent variables

SLIT2 promoter methylation levels measured by real-time quantitative methylation-specific PCR (RT-qMSP)

control variables

ALU methylation used as a reference for relative SLIT2 promoter methylation calculation

controls

No positive or negative controls were explicitly mentioned in the provided information.

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