Human lung cancer-derived cell lines, A549, NCI-H322, H358, H1299, H146, H596, H82, H526, H417, H446, H727, H292, H1155, H157, and H1688 cells were obtained from Curt Harris (NCI), H838 and H1703 cells were from Giuseppe Giaccone (then NCI, currently Georgetown University), and SW620 colon cancer derived cell line was from the DTP (Developmental Therapeutics Program, NCI/DCTD (Division of Cancer Treatments and Diagnostics)) tumor cell lines depository. HeLa and HCT116 cells were purchased from American Type Culture Collection (ATCC).
Most of human cell lines were cultured in RPMI 1640 Medium (LONZA) with or without heat-inactivated fetal bovine serum (FBS: GEMINI BIO), supplemented with penicillin/streptomycin (1:100) or antibiotic/antimycotic solution (10,000 units/ml penicillin G, 10 mg/ml streptomycin sulfate, 25 μg/ml amphotericin B: GEMINI BIO) at 37 ˚C, 5% CO2. HCT116 cells were cultured in McCoy’s 5A Medium (LONZA) instead of RPMI1640 as a medium. For in vitro CCK8 assays, rhSCGB3A2 (1 μg/ml) containing 0.00936 EU (endotoxin unit)/μg of LPS17 (link) was mixed with 100 pg/ml of LPS (10:1 weight ratio for rhSCGB3A2/LPS) and incubated for 10 min at room temperature prior to addition to the culture media.
Most of human cell lines were cultured in RPMI 1640 Medium (LONZA) with or without heat-inactivated fetal bovine serum (FBS: GEMINI BIO), supplemented with penicillin/streptomycin (1:100) or antibiotic/antimycotic solution (10,000 units/ml penicillin G, 10 mg/ml streptomycin sulfate, 25 μg/ml amphotericin B: GEMINI BIO) at 37 ˚C, 5% CO2. HCT116 cells were cultured in McCoy’s 5A Medium (LONZA) instead of RPMI1640 as a medium. For in vitro CCK8 assays, rhSCGB3A2 (1 μg/ml) containing 0.00936 EU (endotoxin unit)/μg of LPS17 (link) was mixed with 100 pg/ml of LPS (10:1 weight ratio for rhSCGB3A2/LPS) and incubated for 10 min at room temperature prior to addition to the culture media.
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