Biliary Specification of hiPSC-Derived Hepatoblasts

hiPSCs were differentiated into Foregut Progenitor cells (FP) as previously described (12 (link), 44 (link)). Bipotent hepatoblasts were generated by culturing FPs in RPMI (Gibco, Invitrogen) + B27 supplemented with SB-431542 (10μM, Tocris Bioscience) and BMP4 (50ng/ml) for 4 days. To induce biliary specification, we cultured hepatoblasts for another 4 days in the presence of RPMI (Gibco, Invitrogen) + B27 supplemented with FGF10 (50ng/ml, Peprotech), activin-A (50ng/ml) and RA (3μM, Sigma-Aldrich).

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Sampaziotis F., de Brito M.C., Madrigal P., Bertero A., Saeb-Parsy K., Soares F.A., Schrumpf E., Melum E., Karlsen T.H., Bradley J.A., Gelson W.T., Davies S., Baker A., Kaser A., Alexander G.J., Hannan N.R, & Vallier L. (2015). Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation. Nature biotechnology, 33(8), 845-852.

Publication 2015

RPMI SB-431542 BMP4 FGF10 activin-A

Activin a Biliary Bmp4 Fgf10 Hipscs Progenitor cells Sb 431542

Corresponding Organization :

Other organizations : Wellcome/MRC Cambridge Stem Cell Institute, Medical Research Council, University of Cambridge, Oslo University Hospital, Cambridge University Hospitals NHS Foundation Trust, Kings Health Partners, Wellcome Sanger Institute

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Variable analysis

independent variables

SB-431542 (10μM, Tocris Bioscience)
BMP4 (50ng/ml)
FGF10 (50ng/ml, Peprotech)
Activin-A (50ng/ml)
RA (3μM, Sigma-Aldrich)

dependent variables

Differentiation of hiPSCs into Foregut Progenitor cells (FP)
Generation of bipotent hepatoblasts
Induction of biliary specification

control variables

RPMI (Gibco, Invitrogen) + B27

controls

No positive or negative controls were explicitly mentioned.

Annotations

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