Culturing Human Chordoma Cell Lines

Human chordoma U-CH1, MUG-Chor1 and JHC7 cell lines were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). U-CH1 and MUG-Chor1 cells were cultured in RPMI-1640 medium (Catalog No. 11875093, Gibco, US) supplemented with an additional 1% l-glutamine (Catalog No. 25030081, Gibco, US), 10% characterized fetal bovine serum (FBS) (Catalog No. 10099141, Gibco, US), 10 units/mL penicillin and 10 mg/mL streptomycin (Catalog No. 10378016, Gibco, US). JHC7 cells were cultured in DMEM: F12 medium (ATCC^® 30-2006™, ATCC, USA) supplemented with 10% FBS, 10 units/mL penicillin and 10 mg/mL streptomycin. To culture U-CH1 and MUG-Chor1 cells, coating buffer (50 μg/mL rat tail type I collagen (Catalog No. 354236, BD Biosciences) was added to the culture flask for 1 h at room temperature prior to adding the cells. The SGC-7901 (a gastric cancer cell line)-shN and sh393 cells and GES-1 (an immortalized gastric epithelial cell line)-shN and sh393 cells were obtained as previously described [32 (link)]. All cells were maintained in humidified incubators at 37 °C with 5% CO₂.