Epigenetic Chromatin Modification Analysis

Gene expression levels were determined as described ^{45 (link)}. For PCRarrays, RNA was converted to cDNA and analyzed on a mouse epigenetic chromatin modification enzymes PCR array platform (PAMM-085, SA Biosciences) following the manufacturer’s protocol. PCR arrays results were analyzed using the RT² Profiler PCR Array Data Analysis (SA Biosciences).

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de Almeida Nagata D.E., Ting H.A., Cavassani K.A., Schaller M.A., Mukherjee S., Ptaschinski C., Kunkel S.L, & Lukacs N.W. (2015). Epigenetic control of Foxp3 by SMYD3 H3K4 histone methyltransferase controls iTreg development and regulates pathogenic T cell responses during pulmonary viral infection. Mucosal immunology, 8(5), 1131-1143.

Publication 2015

PAMM-085 RT2 Profiler PCR Array Data Analysis

Cdna Chromatin Enzymes Epigenetic modification Gene expression Mouse

Corresponding Organization :

Other organizations : University of Michigan–Ann Arbor

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Variable analysis

independent variables

Gene expression levels

dependent variables

Gene expression levels

control variables

Manufacturer's protocol for PCR array platform (PAMM-085, SA Biosciences)

controls

Positive control
Negative control

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