C57BL/6J mice were trained to run for rewards in real and virtual environments29 (link) while recordings were obtained from MEC. Tetrode recordings were performed with a microdrive and headplate assembly that could be used interchangeably for navigation in a real two-dimensional (2D) arena and a virtual 1D track38 . Whole-cell recordings were obtained as described previously29 (link), while mice ran along virtual 1D tracks. Biocytin fills were used to recover cell morphology and determine soma location. A complete description of the methods is available as Supplementary Information.
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Neurophysiology of Spatial Navigation
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Variable analysis
independent variables
- Running in real and virtual environments
- Neural recordings from Medial Entorhinal Cortex (MEC)
- Cell morphology and soma location
- Tetrode recordings performed with a microdrive and headplate assembly
- Whole-cell recordings obtained as described previously
- Positive control: Real two-dimensional (2D) arena
- Negative control: Virtual one-dimensional (1D) track
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