Detailed Staphylococcaceae Strain Identification

Information about the different strains used in this study is provided in Data Set S1 in the supplemental material. Samples were either collected in the framework of routine diagnostic procedures or as part of investigations on a permit issued by the accredited Institutional Animal Care and Use Committee of the International Livestock Research Institute in Nairobi, Kenya (approval no. IACUC 2014.8). The Staphylococcaceae strains were isolated using standard methods (37 ) without any enrichment to select for high MICs and stored for subsequent use at −80°C. Species designation of the various strains was first performed via MALDI-TOF MS analysis and then, once the draft genomes were obtained (see below), confirmed by genomic sequence analysis using the type strain genome server (TYGS) (18 (link)). For MALDI-TOF MS analysis, strains were streaked onto trypticase soy agar with 5% sheep blood (TSA-B; Becton, Dickinson and Co.) and incubated at 37°C overnight. A small amount of colony material was transferred onto a steel target plate, 1 μL of 70% formic acid was added to the colony material, and it was allowed to air dry before 1 μL of HCCA matrix solution (α-cyano-4-hydroxycinnamic acid; Bruker Daltonics) was added. MALDI-TOF MS measurements to identify the species were performed using a Microflex LT instrument (Bruker Daltonics) and the MBT 7854 MSP Library.

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Akarsu H., Liljander A., Younan M., Brodard I., Overesch G., Glücks I., Labroussaa F., Kuhnert P., Perreten V., Monecke S., Drexler J.F., Corman V.M., Falquet L, & Jores J. (2022). Genomic Characterization and Antimicrobial Susceptibility of Dromedary-Associated Staphylococcaceae from the Horn of Africa. Applied and Environmental Microbiology, 88(21), e01146-22.

Publication 2022

HCCA matrix solution Microflex LT instrument MBT 7854 MSP Library

Agar Blood Formic acid Genome Hydroxycinnamic acid Iacuc Library Livestock Maldi ms Mics Routine diagnostic procedures Sequence analysis Sheep Staphylococcaceae Steel Strains Trypticase soy

Corresponding Organization : International Livestock Research Institute

Other organizations : SIB Swiss Institute of Bioinformatics, Friedrich Schiller University Jena, Berlin Institute of Health at Charité - Universitätsmedizin Berlin, Freie Universität Berlin, University of Fribourg

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independent variables

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control variables

Samples were either collected in the framework of routine diagnostic procedures or as part of investigations on a permit issued by the accredited Institutional Animal Care and Use Committee of the International Livestock Research Institute in Nairobi, Kenya (approval no. IACUC 2014.8)
The Staphylococcaceae strains were isolated using standard methods (37 (no link found)) without any enrichment to select for high MICs and stored for subsequent use at −80°C
Species designation of the various strains was first performed via MALDI-TOF MS analysis and then, once the draft genomes were obtained (see below), confirmed by genomic sequence analysis using the type strain genome server (TYGS) (18 (link))
For MALDI-TOF MS analysis, strains were streaked onto trypticase soy agar with 5% sheep blood (TSA-B; Becton, Dickinson and Co.) and incubated at 37°C overnight. A small amount of colony material was transferred onto a steel target plate, 1 μL of 70% formic acid was added to the colony material, and it was allowed to air dry before 1 μL of HCCA matrix solution (α-cyano-4-hydroxycinnamic acid; Bruker Daltonics) was added. MALDI-TOF MS measurements to identify the species were performed using a Microflex LT instrument (Bruker Daltonics) and the MBT 7854 MSP Library

positive controls

None explicitly mentioned

negative controls

None explicitly mentioned

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