Antibodies for Poliovirus Detection

Mouse monoclonal anti-poliovirus VP3, 2B, 2C, and 3A, and rabbit polyclonal antibodies against poliovirus 3B were a gift from prof. Kurt Bienz (University of Bazel, Switzerland) and were partially described in [121 (link)–123 (link)]. Rabbit polyclonal anti-poliovirus 3D antibodies were custom generated by Chemicon and were described in [36 (link)]. Mouse monoclonal anti-dsRNA antibody J2 was from English & Scientific Consulting Kft.
Secondary Alexa dye fluorescent antibody and streptavidin conjugates were from Thermo Fisher, HRP secondary antibody conjugates were from Amersham or KPL. Streptavidin HRP conjugate was from Millipore Sigma (RPN1231).

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Moghimi S., Viktorova E.G., Gabaglio S., Zimina A., Budnik B., Wynn B.G., Sztul E, & Belov G.A. (2022). A Proximity biotinylation assay with a host protein bait reveals multiple factors modulating enterovirus replication. PLOS Pathogens, 18(10), e1010906.

Publication 2022

Streptavidin HRP conjugate

Anti antibodies Anti antibody Antibodies Antibody Dsrna Fluorescent antibody Mouse Mouse poliovirus Poliovirus Rabbit Streptavidin

Corresponding Organization : University of Alabama at Birmingham

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Variable analysis

independent variables

Mouse monoclonal anti-poliovirus VP3, 2B, 2C, and 3A
Rabbit polyclonal antibodies against poliovirus 3B
Rabbit polyclonal anti-poliovirus 3D antibodies
Mouse monoclonal anti-dsRNA antibody J2

dependent variables

Unspecified

control variables

Unspecified

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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