Culturing Hematopoietic Cell Lines

NK-92 parental cells were purchased from the American Type Culture Collection (ATCC) and were cultured with MEM Alpha Medium (1X) + GlutaMAX (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 20% v/v FBS, 1% v/v L-glu, 1% v/v P/S and 100 U/mL IL-2 (Miltenyi Biotec, Bergisch Gladbach, Germany). AML cell lines (NOMO-1, THP-1, U-937) and B-ALL cell lines (KOPN-8, MHH-CALL-4, Nalm-6) were obtained from the German Collection of Microorganisms and Cell Cultures GmbH (DSMZ, Braunschweig, Germany). Transgenic Nalm-6 GFP/Luciferase and U-937 CD19tag/Luciferase were generated as previously described [39 (link),40 (link)]. Leukemia cell lines were cultured with RPMI medium supplemented with 10% v/v FBS, 1% v/v L-glu, 1% v/v P/S (Thermo Fisher Scientific, Waltham, MA, USA) except MHH-CALL-4 which was supplemented with 20% v/v FBS.