P. anserina mycelia were grown on glass slides with a central depression filled with CM agar and incubated for 1 d at 27°C and constant light. Hyphae were visualized and documented using a fluorescence microscope (DM LB/11888011, Leica, Wetzlar, Germany) equipped with the appropriate excitation and emission filters. Light sheet-based fluorescence microscopy (LSFM) and quantification of autophagosomes was performed as described previously.12 (link)
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P. anserina Mycelia Fluorescence Microscopy
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Corresponding Organization : Goethe University Frankfurt
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Variable analysis
independent variables
- Growth medium (CM agar)
- Hyphal morphology and visualization
- Autophagosome quantification
- Incubation temperature (27°C)
- Light conditions (constant light)
- No positive or negative controls were explicitly mentioned.
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