Protocol detail

Inhibition of NF-κB and PI3K Pathways

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RAW 264.7 macrophages were treated with 15μM Bay11-7082 (Cayman Chemical), an inhibitor of κB kinase phosphorylation, for 1 h to inhibit the NF-κB signaling pathway, and then cells were further treated [27 (link)]. RAW 264.7 macrophages were treated with 25μM LY294002 (Cayman Chemical), an inhibitor of PI3K activation [28 (link)], for 1 h, and cells were further treated. Inhibitors were administered prior to LPS and GYY4137 treatments; fresh media was applied following inhibition.

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Cornwell A., Fedotova S., Cowan S, & Badiei A. (2022). Cystathionine γ-lyase and hydrogen sulfide modulates glucose transporter Glut1 expression via NF-κB and PI3k/Akt in macrophages during inflammation. PLOS ONE, 17(12), e0278910.

Publication 2022

Bay11-7082 LY294002

Bay11 7082 Cayman Cells Gyy4137 Inhibit Inhibition Inhibitors Kinase Ly294002 Macrophages Nf κb Phosphorylation Pi3k Signaling pathway

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Variable analysis

independent variables

15μM Bay11-7082 (Cayman Chemical)
25μM LY294002 (Cayman Chemical)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

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