PacBio Genome Sequencing of Altamira Strain

Cells of the A. altamirensis strain C2P003 were cultured in R2 broth for two days at 25 °C and washed two times with 0.3% NaCl. Genomic DNA was extracted by the Genomic-Tip 20 Kit (Qiagen, Hilden, Germany) as described previously [19 (link)]. The fragment size, quantity and quality of DNA were assessed on a 1% agarose gel and with a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). DNA was sequenced using the Pacific Biosciences (PacBio) RS II sequencing platform at Eurofins Genomics (Konstanz, Germany). Sequence reads were de novo assembled using the PacBio hierarchical genome assembly process (HGAP4). The assembling resulted in one contig with an average genome coverage of 124×. The genome sequence was circulated with the Circlator v. 1.5.5 [91 (link)].