Epidermal Sheet Isolation from Mouse Ears

Epidermal sheets were prepared as before^{4 (link)} with slight modification. Briefly, ears were split into dorsal and ventral halves with forceps. Thioglycolic acid-containing Hair Removing Body Cream Epilat (Kracie) was used to remove hair in some experiments. Ear halves were incubated for 15 minutes at 37°C on 3.8% ammonium thiocyanate (Wako Pure Chemical Industries) in phosphate buffer (pH 7.0). Epidermal sheets were manually detached from the dermis under a dissecting microscope (Olympus).

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Adachi T., Kobayashi T., Sugihara E., Yamada T., Ikuta K., Pittaluga S., Saya H., Amagai M, & Nagao K. (2015). Hair follicle-derived IL-7 and IL-15 mediate skin-resident memory T cell homeostasis and lymphoma. Nature medicine, 21(11), 1272-1279.

Publication 2015

ammonium thiocyanate dissecting microscope

Ammonium thiocyanate Body Buffer Dermis Epidermal Forceps Hair Microscope Phosphate Thioglycolic acid

Corresponding Organization :

Other organizations : Keio University, National Institutes of Health, Saitama Medical University, Kyoto University

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Variable analysis

independent variables

Use of Thioglycolic acid-containing Hair Removing Body Cream Epilat (Kracie) to remove hair in some experiments

dependent variables

Epidermal sheet detachment from the dermis

control variables

Incubation time (15 minutes)
Incubation temperature (37°C)
Ammonium thiocyanate concentration (3.8%) in phosphate buffer (pH 7.0)

controls

Not specified
Not specified

Annotations

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