We used PCR to determine the infection status of each line with respect to the endosymbiont, Wolbachia pipientis (Braig et al. 1998 (link); Richardson et al. 2012 (link); Supplemental Text S2). We used DGRP_101 and DGRP_105 as negative controls and DGRP_142 and DGRP_149 as positive controls. We also developed PCR assays to genotype all DGRP lines for insertions of Wolbachia genome at 2R:16,594,660 and 2R:19,117,791 (Supplemental Text S2). We purified PCR products for lines positive for Wolbachia insertions using the Zymo Clean and Concentrator kit (Zymo Research Corporation) and subjected them to Sanger sequencing using the ABI 3730XL platform.
Huang W., Massouras A., Inoue Y., Peiffer J., Ràmia M., Tarone A.M., Turlapati L., Zichner T., Zhu D., Lyman R.F., Magwire M.M., Blankenburg K., Carbone M.A., Chang K., Ellis L.L., Fernandez S., Han Y., Highnam G., Hjelmen C.E., Jack J.R., Javaid M., Jayaseelan J., Kalra D., Lee S., Lewis L., Munidasa M., Ongeri F., Patel S., Perales L., Perez A., Pu L., Rollmann S.M., Ruth R., Saada N., Warner C., Williams A., Wu Y.Q., Yamamoto A., Zhang Y., Zhu Y., Anholt R.R., Korbel J.O., Mittelman D., Muzny D.M., Gibbs R.A., Barbadilla A., Johnston J.S., Stone E.A., Richards S., Deplancke B, & Mackay T.F. (2014). Natural variation in genome architecture among 205 Drosophila melanogaster Genetic Reference Panel lines. Genome Research, 24(7), 1193-1208.
Other organizations :
North Carolina State University, École Polytechnique Fédérale de Lausanne, SIB Swiss Institute of Bioinformatics, Osaka University, Universitat Autònoma de Barcelona, Texas A&M University, European Molecular Biology Laboratory, Baylor College of Medicine, Virginia Tech
Infection status of each line with respect to the endosymbiont, Wolbachia pipientis
Presence of Wolbachia genome insertions at 2R:16,594,660 and 2R:19,117,791
control variables
DGRP_101 and DGRP_105 as negative controls
DGRP_142 and DGRP_149 as positive controls
Annotations
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