Nerve-specific IL-10 receptor knockout in CIPN

Male and female 9-week old WT (JAX#000664) and Il10^−/− (JAX#002251) mice were purchased from Jackson laboratory (Maine). To generate the Avil^Cre+/−;Il10ra^floxflox (Il10ra^DRG-KO) mice, Avil^Cre+/− mice [27 (link),75 (link)] (generously provided by Dr. Hui-Lin Pan, MD Anderson Cancer Center) were bred with Il10ra^floxflox [41 (link)] mice. Both strains were on C57BL/6 background. Experimental animals were obtained from Avil^Cre+/−;Il10ra^floxflox sir crossed with Il10ra^floxflox dam mice. The breeding generated 50% of Avil^Cre+/−;Il10ra^floxflox (Il10ra^DRG-KO) pups and 50% of control Il10ra^floxflox pups. No sex difference was observed in the proportion of each genotype. CIPN was induced by intraperitoneal administration of 2 mg/kg cisplatin (Teva pharmaceuticals) daily for 3 days (identified as days 0, 1 and 2 in figures) as previously described [26 (link)].

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Laumet G., Bavencoffe A., Edralin J.D., Huo X.J., Walters E.T., Dantzer R., Heijnen C.J, & Kavelaars A. (2020). Interleukin-10 resolves pain hypersensitivity induced by cisplatin by reversing sensory neuron hyperexcitability. Pain, 161(10), 2344-2352.

Publication 2020

JAX#000664 cisplatin

Cancer Cisplatin Experimental animals Female Genotype Il10 Intraperitoneal administration Male Mice Pharmaceuticals Sex difference Strains

Corresponding Organization :

Other organizations : Michigan State University, The University of Texas MD Anderson Cancer Center, The University of Texas Health Science Center at Houston

Top 5 similar protocols

Variable analysis

independent variables

Genotype (WT, Il10-/-, Avil^Cre+/-;Il10ra^flox/flox (Il10ra^DRG-KO))
Sex (male, female)

dependent variables

CIPN (Cisplatin-Induced Peripheral Neuropathy)

control variables

Age (9-week-old mice)
Strain (C57BL/6 background)

controls

Positive control: WT mice
Negative control: Il10^-/- mice

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!