The CMV-Gag-Pol murine leukemia virus (MLV) packaging construct, encoding the MLV gag and pol genes, and the MLV-GFP plasmid, encoding an MLV-based transfer vector containing a CMV-GFP internal transcriptional unit, were described previously (see Fig. 1 B; reference 11 (link)). The pCMVΔ8.2 (13 (link)) HIV-1 Gag-Pol packaging construct contains all HIV-1 genes except env, which encodes the envelope glycoproteins, and nef. The HPPT-EF1α-GFP (14 (link)) HIV-1–based transfer vector contains the EF1α internal promoter driving the eGFP marker gene. The gene encoding GFP in this latter vector was replaced by the nlslacZ gene, encoding a nuclear-targeted β-galactosidase, to obtain the HPPT-EF1α-nlslacZ HIV-1–based vector. The phCMV-G (11 (link)) and phCMV-RD (15 (link)) expression vectors encode the vesicular stomatitis virus (VSV) G protein and the feline endogenous virus RD114 glycoproteins, respectively.
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Lentiviral and Retroviral Vector Production
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All 1
Env genes
Feline
G protein
Gene
Gene vector
Glycoproteins
Hiv 1
Murine leukemia virus
Plasmid
Transcriptional
Vectors
Vesicular stomatitis virus
Virus
β galactosidase
Corresponding Organization :
Other organizations : École Normale Supérieure de Lyon, Institut de Biologie de Lille, Institut Pasteur de Lille
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Protocol cited in 14 other protocols
Variable analysis
independent variables
- CMV-Gag-Pol murine leukemia virus (MLV) packaging construct, encoding the MLV gag and pol genes
- MLV-GFP plasmid, encoding an MLV-based transfer vector containing a CMV-GFP internal transcriptional unit
- PCMVΔ8.2 (13) HIV-1 Gag-Pol packaging construct contains all HIV-1 genes except env, which encodes the envelope glycoproteins, and nef
- HPPT-EF1α-nlslacZ HIV-1–based vector (where the gene encoding GFP in the HPPT-EF1α-GFP vector was replaced by the nlslacZ gene, encoding a nuclear-targeted β-galactosidase)
- PhCMV-G expression vector encoding the vesicular stomatitis virus (VSV) G protein
- PhCMV-RD expression vector encoding the feline endogenous virus RD114 glycoproteins
- Measured outcomes not explicitly mentioned
- Kept constant to ensure valid results not explicitly mentioned
- Positive control: Not specified
- Negative control: Not specified
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