Quantifying Microbial Burden in Mouse Eyes

As previously described, harvested eyes from euthanized mice at specific time points were homogenized in 400 µL PBS with sterile 1-mm glass beads (BioSpec Products, Inc., Bartlesville, OK, USA), serially diluted 10-fold in PBS, and plated onto BHI agar plates.²⁰ (link)^,²¹ (link)^,²⁴ (link)^,²⁵ (link)^,²⁸ (link)^,⁴⁴ (link)

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Mursalin M.H., Coburn P.S., Miller F.C., Livingston E.T., Astley R, & Callegan M.C. (2021). C-X-C Chemokines Influence Intraocular Inflammation During Bacillus Endophthalmitis. Investigative Ophthalmology & Visual Science, 62(14), 14.

Publication 2021

sterile 1-mm glass beads

Agar Eyes Mice Sterile

Corresponding Organization : Dean McGee Eye Institute

Top 5 similar protocols

Variable analysis

independent variables

Time points of harvesting eyes from euthanized mice

dependent variables

Bacterial load (colony-forming units) in the harvested eyes

control variables

Homogenization of harvested eyes in 400 µL PBS with sterile 1-mm glass beads
Serial dilution of the homogenized samples in 10-fold increments in PBS
Plating of the diluted samples onto BHI agar plates

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!