Neutrophil Depletion for Bacterial Infection

Neutrophils were depleted using anti-Gr-1 mAb RB6–8C5 (BioXCell). Specifically, starting at one day before bacterial infection, mice were injected i.p. with anti-Gr-1 mAb RB6–8C5 (0.1 mg/day) to deplete neutrophils or with rat IgG as a control. The efficiency of neutrophil depletion in bacterial-infected mice was confirmed by flow cytometry (27 (link)).

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Verma A.K., Bansal S., Bauer C., Muralidharan A, & Sun K. (2020). Influenza infection induces alveolar macrophage dysfunction and thereby enables noninvasive Streptococcus pneumoniae to cause deadly pneumonia. Journal of immunology (Baltimore, Md. : 1950), 205(6), 1601-1607.

Publication 2020

RB6–8C5

Bacterial Bacterial infection Flow cytometry Mice Neutrophil

Corresponding Organization :

Other organizations : University of Nebraska Medical Center

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Variable analysis

independent variables

Treatment with anti-Gr-1 mAb RB6–8C5 (0.1 mg/day)

dependent variables

Efficiency of neutrophil depletion in bacterial-infected mice

control variables

Injection of rat IgG

controls

Positive control: Injection of anti-Gr-1 mAb RB6–8C5 to deplete neutrophils
Negative control: Injection of rat IgG

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