Total yeast DNA was isolated as described previously [23 (link)]. Next, 10 μg of total DNA was digested with BamHI, an enzyme that does not cut within rDNA arrays. Then, PFGE was performed, followed by capillary transfer onto nylon membranes (Sigma-Aldrich) and Southern hybridization with the [α32P] ATP-labeled RDN25-1 probe. Radioactive signals were registered using a FujiFilm FLA-7000 scanner.
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