Real-time Cerebral Blood Flow Measurement

Real-time two-dimensional cerebral blood flow (CBF) was measured using laser speckle flowmetry (LSF, RFLSI III, RWD, China) in sham and BCAS mice 4 weeks postoperation as previously reported (Li C et al., 2022 (link)). Briefly, the mice were anesthetized and placed in a prone position. The skull was exposed by an incision of the skin along the midline of the scalp. Then, the LSF was elevated to an appropriate height above the skull surface. And a whole-brain scan was performed using the LSF. To assess CBF changes, regions of interest (ROIs) were manually selected and the data were analyzed using LSCI_V 1.0.0 software (RWD, China). Body temperature was maintained throughout the experiment.