DNA was extracted for metagenomic sequencing as previously described [18 (link)]. Briefly, DNA was extracted from filtered biomass using a Zymo (Irvine, CA) Research Xpedition Soil/Fecal DNA MiniPrep Extraction kit according to manufacturer instructions. Sequencing libraries were prepared in triplicate using the Nextera XT kit (Illumina Inc., San Diego, CA) and sequenced on the Illumina HiSeq 2500 Rapid Run platform (2 × 250 bp).
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