RNA isolation and qRT-PCR were done as described previously [21 (link)]. Real-time PCR (qPCR) and gene specific primers are detailed in Supplementary Table S2. qPCR reactions were done using 25 ng of cDNA/reaction and 2×iQTM SYBR Green Super mix (Bio-Rad, Hercules, CA, USA) and for 25 cycles, unless described otherwise.
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