Induction of Collagen-Induced Arthritis in Mice

The induction of CIA was described previously (15 (link)). Briefly, male 6 week-old DBA/1J mice (Shanghai Laboratory Animal Center, Chinese Academy of Science) were injected intradermally at the base of the tail with 200 μg bovine CII (Chondrex, USA) emulsified with complete Freund adjuvant (CFA; Chondrex). At day 21, a booster immunization in the same manner was given to it. The animal experiments were performed in accordance with the guidelines approved by Institutional Animal Care and Use Committee of Nanjing Medical University.

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Wang F., Luo A., Xuan W., Qi L., Wu Q., Gan K., Zhang Q., Zhang M, & Tan W. (2019). The Bone Marrow Edema Links to an Osteoclastic Environment and Precedes Synovitis During the Development of Collagen Induced Arthritis. Frontiers in Immunology, 10, 884.

Publication 2019

DBA/1J mice bovine CII

Booster immunization Bovine Chinese Chondrex Dba mice Freund adjuvant Institutional animal care and use committee Laboratory animal Male Tail

Corresponding Organization : Jiangsu Province Hospital

Other organizations : Nanjing Traditional Chinese Medicine Hospital

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Variable analysis

independent variables

Injection of 200 μg bovine CII emulsified with complete Freund adjuvant (CFA) intradermally at the base of the tail
Booster immunization of 200 μg bovine CII emulsified with CFA at day 21

dependent variables

Outcome of the CIA induction (not explicitly mentioned)

control variables

Male 6 week-old DBA/1J mice
Animal experiments performed in accordance with the guidelines approved by Institutional Animal Care and Use Committee of Nanjing Medical University

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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