Antibody Characterization for Xenopus Proteins

α-PCNA (P8825) and α-HIS (H1029) were purchased from Sigma; α-RIF1 (A300-568A) was purchased from Bethyl; α-PP1α (sc-271762) and α-PP2α (sc-56950) were purchased from Santa Cruz Biotechnology.
Affinity purified α-Cdc45, α-Psf2 (30 (link)), α-Mcm4 antibodies (31 (link)), α-Mcm7 (32 (link)), α-RecQ4 and α-TopBP1 antibodies were previously described (33 (link)). Similarly, α-GINS (34 (link)), α-Treslin and α-MTBP were described in (18 (link)).
Xenopus full-length DONSON protein was purified as described above and antibodies raised against such prepared antigens in sheep or rabbit. The resulting antibody sera was purified in-house against the purified antigen. The specificity of each new antibody is presented in Supplementary Figure S1.

Free full text: Click here

Kingsley G., Skagia A., Passaretti P., Fernandez-Cuesta C., Reynolds-Winczura A., Koscielniak K, & Gambus A. (2023). DONSON facilitates Cdc45 and GINS chromatin association and is essential for DNA replication initiation. Nucleic Acids Research, 51(18), 9748-9763.

Publication 2023

α-PCNA (P8825) α-HIS (H1029)

Antibodies Antibody Antibody specificity Antigen Mcm4 Pcna Protein Rabbit Recq4 Sera Sheep Xenopus

Corresponding Organization :

Other organizations : University of Birmingham

Top 5 similar protocols

Variable analysis

independent variables

α-PCNA (P8825) and α-HIS (H1029) were purchased from Sigma
α-RIF1 (A300-568A) was purchased from Bethyl
α-PP1α (sc-271762) and α-PP2α (sc-56950) were purchased from Santa Cruz Biotechnology
Affinity purified α-Cdc45, α-Psf2, α-Mcm4, α-Mcm7, α-RecQ4 and α-TopBP1 antibodies
α-GINS, α-Treslin and α-MTBP antibodies
Xenopus full-length DONSON protein

dependent variables

Measured outcomes related to the usage of the purchased antibodies and purified Xenopus DONSON protein

control variables

The specificity of each new antibody was presented in Supplementary Figure S1

Annotations

Based on most similar protocols

The iCBT treatment in the Input protocol was previously tested and used in regular care in Sweden (Protocol 1). This suggests the treatment has been validated and optimized for performance in the targeted population.

The Input protocol included a pilot group of 10 patients to test the condensed version of the iCBT treatment before the main study (Input protocol). This validation step helps ensure the condensed version maintains the same effectiveness as the full-text version.

The Input protocol and Protocols 1-4 all used therapist guidance and feedback as part of the iCBT treatment, which can enhance the accuracy and effectiveness of the treatment approach compared to unguided iCBT (Protocol 5).

The Input protocol and Protocol 1 both recommended participants complete one module per week, which provides a standardized timeframe for the intervention that could improve reproducibility (Input protocol, Protocol 1).

Protocols 2-3 specified the iCBT treatment was delivered through a secure, encrypted web-based platform, which is an important control variable to ensure data privacy and treatment fidelity (Protocol 2, Protocol 3).

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!