Immunostaining and Microscopy Analysis of Cryosections
Cryosections were fixed in acetone for 10 min. Immunostaining and analysis of cryosections were performed as described previously [28 (link)]. The following antibodies were used: anti-CD3 (1:150; Bio-Rad, United States, Hercules), anti-F4/80 (1:100; Bio-Rad), anti-MBP (1:250; Sigma‒Aldrich), and anti-SMI312 (1:250; Biolegend) combined with Alexa Fluor 555-labeled anti-rat IgG and Alexa Fluor 488-labeled anti-mouse IgG secondary antibodies (1:400; Invitrogen, United States, Waltham). Analysis was carried out using a Nikon Eclipse 80i microscope and ImageJ software.
Other organizations :
Hasselt University, KU Leuven, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, University of Wisconsin–Madison, University of Graz, BioTechMed-Graz, Netherlands Metabolomics Centre, Leiden University Medical Center
Immunostaining and analysis of cryosections were performed as described previously [28 (link)]
controls
Positive controls: Not specified
Negative controls: Not specified
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