NETest and CgA Biomarker Collection

Blood samples for the NETest were collected in 10.8mg K₂EDTA tubes (BD Vacutainer Venous Blood Collection Tubes, BD Diagnostics). Aliquots of whole blood were stored at -80°C within 2 hrs of collection (samples immediately stored on ice/4°C after sampling) per standard molecular diagnostics protocols [24 (link)]. Blood samples for CgA were collected at the same time point in PPT plasma preparations tubes. Matched tumor tissue samples were available in seven cases. Tissue (n = 7) were collected at the time of surgery [25 (link)]. Samples were snap frozen in liquid nitrogen. Deidentified samples were sent to Wren Laboratories for RNA isolation and NETest PCR.

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Kidd M., Drozdov I.A., Matar S., Gurunlian N., Ferranti N.J., Malczewska A., Bennett P., Bodei L, & Modlin I.M. (2019). Utility of a ready-to-use PCR system for neuroendocrine tumor diagnosis. PLoS ONE, 14(6), e0218592.

Publication 2019

BD Vacutainer Venous Blood Collection Tubes

Blood Diagnostics Frozen Isolation Molecular diagnostics Nitrogen Plasma Tissue Tumor Venous Wren

Corresponding Organization : Yale University

Top 5 similar protocols

Variable analysis

independent variables

Collection of blood samples for NETest
Collection of blood samples for CgA
Collection of tumor tissue samples

dependent variables

NETest PCR
RNA isolation

control variables

Blood samples collected in 10.8mg K2EDTA tubes
Aliquots of whole blood stored at -80°C within 2 hrs of collection
Blood samples for CgA collected at the same time point in PPT plasma preparations tubes
Tumor tissue samples collected at the time of surgery
Tumor tissue samples snap frozen in liquid nitrogen
Deidentified samples sent to Wren Laboratories

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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