Immunohistochemical Biomarker Validation Protocol

All IHC was performed in a hybrid laboratory (Northern Ireland Molecular Pathology Laboratory) that has UK Clinical Pathology Accreditation. Internally validated biomarker conditions, which followed UK-NEQAS guidelines (CD3 and CD8) or were based on expected performance from the literature (p53 and PD-L1), were as follows: CD3 (clone 2GV6 Ventana BenchMark; CC1 32 minutes, Optiview detection), CD8 (clone c8/144B, Dako: Leica Bond III, ER2 20 mins, 1/50, polymer detection), p53 (clone DO-7, Dako, ER2 30 mins, 1/100, polymer detection), PD-L1 (clone SP142, Ventana BenchMark, CC1 24 mins, optiview detection).

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Bankhead P., Loughrey M.B., Fernández J.A., Dombrowski Y., McArt D.G., Dunne P.D., McQuaid S., Gray R.T., Murray L.J., Coleman H.G., James J.A., Salto-Tellez M, & Hamilton P.W. (2017). QuPath: Open source software for digital pathology image analysis. Scientific Reports, 7, 16878.

Publication 2017

Biomarker Clone Hybrid Pd l1 Polymer

Corresponding Organization :

Other organizations : Queen's University Belfast, Belfast Health and Social Care Trust

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Variable analysis

independent variables

Biomarker conditions (CD3, CD8, p53, PD-L1)

dependent variables

Measured outcomes of biomarker expression

control variables

IHC was performed in a hybrid laboratory with UK Clinical Pathology Accreditation
Biomarker conditions followed UK-NEQAS guidelines (CD3 and CD8) or were based on expected performance from the literature (p53 and PD-L1)

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

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