Panc1 Cell Migration Assay using xCELLigence DP

The xCELLigence^® DP system (ACEA Biosciences, San Diego, CA, USA, distributed by OLS, Bremen, Germany) was employed for recording random cell migration of Panc1 cells. CIM plates-16 were prepared according to the instruction manual and previous descriptions [3 (link),14 (link)]. The underside of the upper chambers of the CIM plate-16 was coated with 30 μL of collagen I (400 μg/mL). In all assays, RPMI with 1% fetal bovine serum was present in both the upper and lower chambers of each well of a CIM plate-16. The upper chamber of each well was loaded with 50,000–60,000 cells immediately after the addition of 5 ng/mL TGF-β1 to the cell suspensions. Data acquisition was done at intervals of 15, 30, or 60 min and analyzed with RTCA software (ACEA).

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Ungefroren H., Otterbein H., Fiedler C., Mihara K., Hollenberg M.D., Gieseler F., Lehnert H, & Witte D. (2019). RAC1B Suppresses TGF-β1-Dependent Cell Migration in Pancreatic Carcinoma Cells through Inhibition of the TGF-β Type I Receptor ALK5. Cancers, 11(5), 691.

Publication 2019

xCELLigence® DP system RTCA software

Assays Cell Cell migration Collagen i Fetal bovine serum Rtca Tgf β1

Corresponding Organization : University Hospital Schleswig-Holstein

Other organizations : University of Calgary

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Variable analysis

independent variables

Addition of 5 ng/mL TGF-β1 to the cell suspensions

dependent variables

Random cell migration of Panc1 cells

control variables

RPMI with 1% fetal bovine serum present in both the upper and lower chambers of each well of a CIM plate-16
Underside of the upper chambers of the CIM plate-16 coated with 30 μL of collagen I (400 μg/mL)
50,000–60,000 cells loaded into the upper chamber of each well

positive controls

None specified

negative controls

None specified

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