Nardochinoid B (NAB) (Figure 1) (HPLC purity >98%) was provided by the Institute of Traditional Chinese Medicine and Natural Products, Jinan University (Guangzhou, China). Dimethyl sulfoxide (DMSO) (Sigma, Cat. No. D2625, St. Louis, MO, USA) was used to dissolve the NAB powder to give a stock solution of 30 mM concentration. Lipopolysaccharide (LPS), dexamethasone (DEX), and sulforaphane (SFN) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Antibodies to iNOS [57 (link)], COX-2 [58 (link)], heme oxygenase (HO)-1 [58 (link)], Nrf2 [18 (link)], p-p65 [18 (link)], p-p38 [18 (link)], and phospho-extracellular regulated protein kinases (p-ERK) [18 (link)] were obtained from Cell Signaling Technology (Boston, MA, USA). Antibodies to β-actin and laminin B1 [18 (link)] were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibody to α-tubulin [18 (link)] was from Sigma Chemical Co. (St. Louis, MO, USA). The secondary antibodies for Western blot were from Li-COR Biotechnology (Lincoln, NE, USA). ELISA kits for IL-1β, IL-6, and TNF-α was from eBioscience (eBioscience, Inc., San Diego, CA, USA). ELISA kit for PGE2 was from Cayman Chemical (Cayman Chemical, Ann Arbor, MI, United States). The nitric oxide (NO) production level was measured by a Griess Reagent System kit, which was obtained from Promega Corporation (Madison, WI, USA).
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