Analytical Standards for Steroidal Glycosides

Analytical standards including tomatidine (Sigma-Aldrich, contains dehydrotomatidine as impurity), α-tomatine (Carbosynth USA, contains dehydrotomatine as impurity), α-solamargine (ChemFaces) were dissolved in methanol at a concentration of 1 mg/ml. Hydroxytomatine used as substrate in recombinant assays during screening of BAHD candidates (Fig. 2b) was isolated and purified from leaves of GAME31-overexpressing transgenic tomato plants as described earlier^{11 (link)}.

Free full text: Click here

Sonawane P.D., Gharat S.A., Jozwiak A., Barbole R., Heinicke S., Almekias-Siegl E., Meir S., Rogachev I., Connor S.E., Giri A.P, & Aharoni A. (2023). A BAHD-type acyltransferase concludes the biosynthetic pathway of non-bitter glycoalkaloids in ripe tomato fruit. Nature Communications, 14, 4540.

Publication 2023

tomatidine α-solamargine

Assays Dehydrotomatine Methanol Solamargine Tomatidine Tomatine Tomato Transgenic plants

Corresponding Organization : Max Planck Institute for Chemical Ecology

Other organizations : Weizmann Institute of Science

Top 5 similar protocols

Variable analysis

independent variables

Tomatidine (Sigma-Aldrich, contains dehydrotomatidine as impurity)
α-tomatine (Carbosynth USA, contains dehydrotomatine as impurity)
α-solamargine (ChemFaces)

dependent variables

Not explicitly mentioned

control variables

Methanol concentration (1 mg/ml)

controls

Positive control: Hydroxytomatine isolated and purified from leaves of GAME31-overexpressing transgenic tomato plants
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!