Immunofluorescence and Immunohistochemistry Assays
Immunofluorescence and immunohistochemistry assays were performed as previously described [2 (link), 10 (link)]. The following primary antibodies were used: anti-Cx43 (Sigma-Aldrich, C6129), anti-collagen II (Invitrogen, Thermo Fisher Scientific, MA5-12789), anti-Ki-67 (BD, 550609), anti-Twist-1 (sc-81417) and anti-NF-κB (sc-8008) from Santa Cruz Biotechnology. Goat anti-rabbit FITC-conjugated (F-2765, Invitrogen, Thermo Fisher Scientific) and goat anti-mouse Alexa 594-conjugated (A-11032, Invitrogen, Thermo Fisher Scientific) secondary antibodies were used.
Varela-Eirín M., Carpintero-Fernández P., Sánchez-Temprano A., Varela-Vázquez A., Paíno C.L., Casado-Díaz A., Continente A.C., Mato V., Fonseca E., Kandouz M., Blanco A., Caeiro J.R, & Mayán M.D. (2020). Senolytic activity of small molecular polyphenols from olive restores chondrocyte redifferentiation and promotes a pro-regenerative environment in osteoarthritis. Aging (Albany NY), 12(16), 15882-15905.
Other organizations :
Instituto de Investigación Biomédica de A Coruña, Servicio Gallego de Salud, Universidade da Coruña, Instituto Ramón y Cajal de Investigación Sanitaria, Hospital Universitario Ramón y Cajal, Hospital Universitario Reina Sofía, University of Córdoba, Instituto Maimónides de Investigación Biomédica de Córdoba, Wayne State University, University College Dublin, Complejo Hospitalario Universitario de Santiago, Complexo Hospitalario Universitario A Coruña, Universidade de Santiago de Compostela
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