For live imaging, wing imaginal discs and their associated trachea were prepared following Roy et al. (2014) (link). Images were obtained as previously described (Du et al., 2018a (link)) using a Leica SP5X with HyD detector or an CSUX1 Yokogawa spinning disc confocal equipped with an Andor iXon897 EMCCD camera. The images were processed and analyzed with Fiji. Maximum-intensity projections of sections were shown for most images. All images were obtained using 40× objective in the microscopes, except for Fig. 8 (J–N) , which used a 20× objective. All XZY images were obtained using the Leica SP5X with a 40× objective for S2 cells and 20× objective for wing discs.
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Live Imaging of Wing Imaginal Discs
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Corresponding Organization : University of Maryland, College Park
Other organizations : University of California, San Francisco
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Variable analysis
independent variables
- Imaging method (Leica SP5X with HyD detector or CSUX1 Yokogawa spinning disc confocal with Andor iXon897 EMCCD camera)
- Wing imaginal discs and their associated trachea
- S2 cells
- Wing discs
- Imaging objective (40x for most images, 20x for Fig. 8 (J-N))
- Image processing and analysis (Fiji)
- None explicitly mentioned
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