Levels of total cholesterol, LDL-C, complete blood cell count, serum triglycerides, HDL-C, albumin, blood urine nitrogen (BUN), uric acid, and glucose were measured from fasting blood samples 30 (link). The Jaffe method was used to calculate serum creatinine. Hemoglobin A1c was measured using an automated analyzer (HLC-723G8, Tosoh Corp., Tokyo, Japan). CK-MB and troponin I serum levels were measured using chemiluminescent microparticle immunoassays. Total leukocyte count and the lymphocyte, neutrophil and monocytes proportions were measured using an automated cell counter (XE-2100 Hematology Alpha Transportation System; Sysmex, Kobe, Japan). Absolute leukocyte subtype counts were calculated as the product of its proportion and total leukocyte count. The CKD-EPI formula was utilized to determine estimated glomerular filtration rate (eGFR) 31 (link). An immunochemistry system (Beckman Coulter IMMAGE) was used to evaluate plasma levels of high-sensitivity C-reactive protein (hs-CRP). The detection limit was 0.2 mg/L, and all measurements were made twice.
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