Inducing Midgut Damage in Anopheles

To induce chemical damage in the midgut, 3- to 4 day-old females of An. stephensi HP10 hindgut line were fed with 10% Karo syrup solution supplemented with 25 µg/ml Bleomycin (Bleomycin sulfate, anticancer and antibiotic agent, ab142977, Abcam, Cambridge, UK) for two days^{25 (link)}. The fresh solution was offered every day of the treatment. After two days, females were artificially fed with 10% BSA saline solution to distend the midgut tissue as described above.

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Barletta A.B., Smith J.C., Burkart E., Bondarenko S., Sharakhov I.V., Criscione F., O’Brochta D, & Barillas-Mury C. (2024). Mosquito midgut stem cell cellular defense response limits Plasmodium parasite infection. Nature Communications, 15, 1422.

Publication 2024

Bleomycin

Corresponding Organization : National Institutes of Health

Other organizations : Virginia Tech, University of Maryland, College Park

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Variable analysis

independent variables

Feeding 3- to 4 day-old females of An. stephensi HP10 hindgut line with 10% Karo syrup solution supplemented with 25 µg/ml Bleomycin for two days

dependent variables

Chemical damage in the midgut

control variables

Age of females (3- to 4 day-old)
Mosquito strain (An. stephensi HP10 hindgut line)
Duration of treatment (two days)

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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