Radiolabeled Isoprenoid Biosynthesis in Plasmodium falciparum
Corresponding Organization : Centro de Investigación Biomédica en Red
Other organizations : Universidade Federal de São Carlos, University of Tübingen, University of Eastern Finland, Instituto de Pesquisas Tecnológicas
Variable analysis
- Concentration of AlbuMAX I Lipid-Rich BSA in RPMI-1640 medium
- Concentration of dolichol and dolichyl-P 13–21
- Concentration of [1-(n)-3H] GGOH, [1-(n)-3H] FOH, and L-[4,5-14C (U)] isoleucine
- Concentration of fosmidomycin sodium salt hydrate, clindamycin hydrochloride, GGOH, and other non-radiolabelled prenols
- Measurement of SYBR Green I nucleic acid gel stain and SYTO 11 fluorescence
- Measurement of polyprenyl phosphates obtained by mild acid treatment of the respective commercial pure pyrophosphates
- Use of a Cre-LoxP P. falciparum NF54 strain
- Use of erythrocytes and CPDA-1 treated hospital-grade plasma bags for transfusions (AB+)
- Polyprenyl phosphates obtained by mild acid treatment of the respective commercial pure pyrophosphates (Sigma; Sigma codes F6892 and G6025)
- Not explicitly mentioned
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