Auranofin (1 μM) was incubated with liver microsomes from human or mouse (0.5 mg/ml) in 0.1 M phosphate buffer. The amount of auranofin remaining after 30 min incubation at 37 °C was determined using LC–MS/MS (Triple Quad 5500, Applied Biosystems, Foster City, CA, USA).
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Auranofin Metabolism in Liver Microsomes
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Corresponding Organization :
Other organizations : Seoul National University, Hanyang University, Daegu-Gyeongbuk Medical Innovation Foundation, Chosun University
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Variable analysis
independent variables
- Incubation with auranofin (1 μM)
- Amount of auranofin remaining after 30 min incubation at 37 °C
- Incubation with liver microsomes from human or mouse (0.5 mg/ml)
- Incubation in 0.1 M phosphate buffer
- Incubation time of 30 min
- Incubation temperature of 37 °C
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