Protein Immunoblotting Analysis of Cell Lysates

Cell lysates were prepared as described (Hasegawa, et al 2016 (link), Yin, et al 2010 ). Soluble proteins were analysed by immunoblotting with anti-MUC1-C (NeoMarkers, Fremont, CA, USA) anti-β-catenin (Calbiochem, San Diego, CA, USA), anti-MYC (Abcam, Cambridge, MA, USA), anti-TCF4, anti-PKCδ, anti-MCL-1 (Santa Cruz, Dallas, TX, USA), anti-β-actin (Sigma-Aldrich, St. Louis, MO, USA), anti-TIGAR, anti-CRBN (Abcam, Cambridge, MA, USA), anti-CD44, anti-poly ADP ribose polymerase (PARP), and anti-BCL-XL (BCL2L1) (Cell Signaling, Danvers, MA, USA). Immune complexes were detected with horseradish peroxidase-conjugated secondary antibodies and enhanced chemiluminescense (GE Healthcare, Piscataway, NJ, USA).